Near isogenic lines pdf files

The complex quantitative nature of droughtrelated traits is a major constraint to breed tolerant wheat varieties. Multiple nearisogenic lines targeting a qtl hotspot of. Abstractto exploit the genetic mechanism of cold tolerance in rice, cold tolerant nearisogenic lines nils. Nineteen genomic regions, identified by restriction. Tortillas were prepared from each deletion line and the parent lines. Near isogenic lines nils are derived via repeated backcrossing of genetically distinct parental lines, most commonly with the aim of transferring a single chromosomal region of the donor parent. Pdf qtl mapping with nearisogenic lines in maize researchgate. Application of a library of near isogenic lines to understand context dependent expression of qtl for grain. Nearisogenic lines nils a good way of validating a putative qtl is by creating a nearisogenic line nil. Comparison of near isogenic lines of wheat showed that the wheat resistance genes is localized within a segment of the short arm of chromosome 2n from aegilops ventricosa zhuk. Pdf nearisogenic lines nils constitute valuable tools in genetic investigations and plant breeding programs. Pdf the development of near isogenic lines nils through repeated backcrossing of genetically distinct parental lines is rather. The progeny with the traitofinterest are then selected for backcrossing to the reference line. Each of the 89 nils, the control line, tx303, and b73 was test crossed to the inbred line mo17 for evaluation.

An aegilops ventricosa translocation confers resistance. The relationships between development and low temperature. Growth, reproduction, and superoxide dismutase activity were unaffected both in the petridish and soil microcosm experiment. Sixteen nearisogenic lines nils carrying a marker gene were produced by the recurrent backcrossing method in the genetic background of. Nearisogenic lines nils integrated breeding platform.

Registration of four nearisogenic soybean lines of g0032 for resistance to asian soybean rust zachary r. The control line was derived from the same cross and backcrossing procedures as described above, but was homozygous for the b73 allele at each of the 19 rflp loci. Identification of molecular markers linked to leaf rust. Backcross populations and near isogenic lines article pdf available in methods in molecular biology clifton, n. Application of a library of near isogenic lines to understand context. To develop the nil mapping population, cross of a donor line with a trait of interest and a reference line is initiated. Characterization and identification of cold tolerant near. Isogenic definition is characterized by essentially identical genes.

We characterized a set of isogenic genetic stocks representing single and pairwise substitutions of spring alleles at the. Production of nearisogenic lines and marked monosomic lines in. Nearisogenic wheat lines in which one or more of these loci were absent or deleted were used. What is difference between isogenic and near isogenic line. Using the marker identified for that qtl, backcrosses are made to the recurrent parent usually the cultivated variety. The elimination of certain hmwgs alleles alter distinct but critical aspects of tortilla quality such as diameter, shelf stability, and overall quality.

Although nearisogenic lines nils can standardize genetic backgrounds among individuals, it has never. The nearisogenic lines nils inbred lines are usually developed for qtl mapping purpose. Cucumis melo, introgression line, qtl, g e interaction, heritability abstract. To develop the nil mapping population, cross of a donor line with a trait of interest and a reference. This is a new line that differs from its parent in only one genomic location. Use of nearisogenic wheat lines to determine the glutenin. An additional file shows the details of experimental conditions in more detail see additional file 1. Identification of molecular markers linked to leaf rust resistance genes in wheat and their detection in the local nearisogenic line. The development and application of nearisogenic lines for. The amylose content, size distribution, and processing properties of isolated starch granules were compared between eight near.

Use of nearisogenic lines derived by backcrossing or selfing to map. Nearisogenic lines nils with single blast resistance genes were developed by backcrossing four donor cultivars to the recurrent parent. In arabidopsis recombinant inbred line ril populations are widely used for quantitative trait locus qtl analyses. Nearisogenic lines nils are a valuable resource for detecting linkages between qualitative trait loci and molecular markers. Estimatingthegeneticarchitectureoffruitquality traits in. Inheritance of blast resistance in nearisogenic lines of rice. The effects of bt and near isogenic lines of rice were studied in two laboratory experiments. Chennav translocated into common wheat triticum aestivum l. Starch granule size distribution in wheat endosperm. Development of nearisogenic lines in a parthenogenetically. Wl711 developed at the school of biotechnology were used along with the donor and the recurrent parents for identifying rapd markers linked to the two genes. Registration of four nearisogenic soybean lines of g00. Development of a nearisogenic line population of arabidopsis. Pdf a set of 89 nearisogenic lines nils of maize was created using marker assisted selection.